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Part:BBa_K5423002:Design

Designed by: Kyle Cavanagh   Group: iGEM24_ANU   (2024-10-02)


mScarlet3 dCas9 gRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5
    Illegal BsaI.rc site found at 41


Design Notes

Needed to target mScarlet3, without targeting E. coli genome


Source

Bradley, R. W. An easy-to-use CRISPRi plasmid tool for inducible knockdown in E. coli. Biotechnol. Rep. 32, e00680 (2021). Sponsors

References